Translational_Unit
Part:BBa_K3208000
Designed by: Slavil Peykov Group: iGEM19_Bulgaria (2019-10-16)
DAMP4-based expression unit for antimicrobial peptide production
DAMP4-based expression unit for antimicrobial peptide production. This part provides the DAMP4 fusion partner that allows expression and purification of toxic AMPs in E. coli. Two Eco31I sites allow easy cloning of new short peptide coding sequences.
For DAMP4 details see:
DOI: 10.1007/s00253-018-9319-4
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 291
Illegal BamHI site found at 316 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 388
Illegal BsaI.rc site found at 301
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Categories
Parameters
None |