Translational_Unit

Part:BBa_K3208000

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-16)


DAMP4-based expression unit for antimicrobial peptide production


DAMP4-based expression unit for antimicrobial peptide production. This part provides the DAMP4 fusion partner that allows expression and purification of toxic AMPs in E. coli. Two Eco31I sites allow easy cloning of new short peptide coding sequences.


For DAMP4 details see:

DOI: 10.1007/s00253-018-9319-4



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 291
    Illegal BamHI site found at 316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 388
    Illegal BsaI.rc site found at 301


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